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O-propargyl-puromycín

3279

10.01.2012

We have previously reported the co-localization of O-propargyl-puromycin (OP-Puro) with SUMO-2/3 and ubiquitin at promyelocytic leukemia-nuclear bodies (PML-NBs) in the presence of the proteasome inhibitor MG132, implying a role for the ubiquitin family in sequestering OP-puromycylated immature polypeptides to the nucleus during impaired proteasome activity. O Propargyl Puromycin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Product Name. Type. HY-15680. O-Propargyl-Puromycin.

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PML-NBs: 3 : 2016: Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition. PML-NBs: 4 : 2012: Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. O-Propargyl-Puromycin Cat. No.: HY-15680 CAS No.: 1416561-90-4 Batch No.: 25014 Chemical Name: Adenosine, 3'-[[(2S)-2-amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyl-PHYSICAL AND CHEMICAL PROPERTIES Molecular Formula: C24H29N7O5 Molecular Weight: 495.53 Storage: Powder -20°C 3 years 4°C 2 years In solvent -80°C 10.01.2012 Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label the C-termini of nascent polypeptide chains (NPCs). Following affinity purification via a click reaction, OPP allows for a proteomic analysis of NPCs. O-propargyl-puromycin [MeSH concept] Semantic type(s) Antibiotic [Semantic type] Nucleic Acid, Nucleoside, or Nucleotide [Semantic type] Keyword's position in hierarchy(ies) : You can consult : All resources. or only the major. or use the search engine.

In this study, using O-propargyl-puromycin (OP-Puro), the distribution of puromycylated polypeptides was assessed in HeLa cells by click chemistry. Under standard culture conditions, OP-Puro signals were detected in the cytoplasm and nucleus with the highest concentrations in the nucleolus.

Stability: ≥1 year at -20°C Laboratory Procedures Prior to use, thaw the O-propargyl-puromycin Stock Solution at room temperature. Briefly spin the vial at 1,000 x O Propargyl Puromycin.

Does anyone know whether "O-propargyl-puromycin", an isotope-free material to Stoleru, D., and Salic, A. 2012. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin.

OPP can conjugate with nascent polypeptide chains thus constituting a covalent form. OPP O propargyl puromycin is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine containing media When used with Click iT Plus OPP Alexa Fluor Protein Synthesis assays or Alexa Fluor Picolyl Azide toolkits OPP provides a fast sensitive and non radioactive method for the detection of protein synthesis using fluorescence microscopy high content … O-propargyl-puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor.

O-propargyl-puromycín

O-Propargyl-Puromycin | C24H29N7O5 | CID 71576433 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more. OPP (O-propargyl-puromycin) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine-containing media. When used with Click-iT Plus OPP Alexa Fluor Protein Synthesis assays or Alexa Fluor Picolyl Azide toolkits, OPP provides a fast, sens In Vitro. O-propargyl-puromycin can be used to image and affinity-purify nascent proteins in cells and in animals. O-propargyl-puromycin inhibits protein synthesis, both in reticulocyte lysates and in cultured cells, displaying a potency two- to threefold lower than that of unmodified puromycin.

O-Propargyl-puromycin I Cat# 1407 I Revised 03/28/2019 O-Propargyl-puromycin Product No.: Product Name: Alternative name: 1407 O-Propargyl-puromycin . OPP. Structure: N N N N N O NH OH HO O NH. 2. O. Molecular Weight: Chemical Composition: CAS Number: … O-propargyl-puromycin(cas 1416561-90-4) is an alkyne analog of puromycin, is a potent protein synthesis inhibitor. O-propargyl-puromycin. Known as: OP-puro National Institutes of Health Create Alert. Related topics. Related topics 2 relations.

OPP (O-propargyl-puromycin) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine-containing media. When used with Click-iT Plus OPP Alexa Fluor Protein Synthesis assays or Alexa Fluor Picolyl Azide toolkits, OPP provides a fast, sens In Vitro. O-propargyl-puromycin can be used to image and affinity-purify nascent proteins in cells and in animals. O-propargyl-puromycin inhibits protein synthesis, both in reticulocyte lysates and in cultured cells, displaying a potency two- to threefold lower than that of unmodified puromycin. O-propargyl-puromycin forms covalent conjugates with O-Propargyl-Puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor.

O-propargyl-puromycín

Related topics. Related topics 2 relations. Broader (1) Puromycin. analogs & derivatives.

OP-puro is incorporated into nascent polypeptide chains where it forms covalent adducts and terminates chain elongation on the ribosome. It has been used in combination with fluorescent azides to image nascent proteins in various cells. An alkyne derivative of puromycin used to supervise newly protein synthesis, named O-propargyl-puromycin (OPP), is a potent protein synthesis inhibitor that stopping translation through be incorporated into the C-terminus of translating polypeptide chains. OPP can conjugate with nascent polypeptide chains thus constituting a covalent form. OPP O propargyl puromycin is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine containing media When used with Click iT Plus OPP Alexa Fluor Protein Synthesis assays or Alexa Fluor Picolyl Azide toolkits OPP provides a fast sensitive and non radioactive method for the detection of protein synthesis using fluorescence microscopy high content … O-propargyl-puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor. O-propargyl-puromycine (OPP) O-propargyl-puromycin (OPP) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins, which are rapidly turned over by the proteasome and can be visualized or captured by copper (I)-catalyzed azide-alkyne cycloaddition. O-Propargyl-Puromycin is a potent inhibitor of protein synthesis.

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O-Propargyl-puromycin (OPP) is cell-permeable alkyne analog of the protein synthesis inhibitor puromycin (Cat. No. 1860) that efficiently incorporates into newly translated proteins in complete methionine-containing media.

OPP (O-propargyl-puromycin) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine-containing media. When used with Click-iT Plus OPP Alexa Fluor Protein Synthesis assays or Alexa Fluor Picolyl Azide toolkits, OPP provides a fast, sens In Vitro. O-propargyl-puromycin can be used to image and affinity-purify nascent proteins in cells and in animals. O-propargyl-puromycin inhibits protein synthesis, both in reticulocyte lysates and in cultured cells, displaying a potency two- to threefold lower than that of unmodified puromycin. O-propargyl-puromycin forms covalent conjugates with O-Propargyl-Puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor. In Vitro.